https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15038 = 300) were assessed via scanning and transmission electron microscopy (SEM and TEM), and compared to control populations. Our analyses found that prolonged exposure to all treatments resulted in the subsequent formation of SCV phenotypes. Observed SCVs manifested as minute colonies with reduced haemolysis and pigmentation (NaCl, pH and 4 degrees C treatments), or complete lack thereof (antibiotic treatments). SEM comparison analyses revealed significantly smaller cell sizes for SCV populations except in S. aureus and S. epidermidis 10% NaCl, and S. epidermidis 4 degrees C (p<0.05). Shifts in population distribution patterns were also observed with distinct sub-populations of smaller cells appearing for S. epidermidis, and S. lugdunensis. TEM analyses revealed significantly thicker cell-walls in all treatments and species except S. lugdunensis exposed to 4 degrees C. These findings suggest that staphylococci adapted to environmental stresses by altering their cell size and wall thickness which could represent the formation of altered phenotypes which facilitate survival under harsh conditions. The phenotypic response was governed by the type of prevailing environmental stress regime leading to appropriate alterations in ultra-structure and size, suggesting downstream changes in gene expression, the proteome, and metabolome.]]> Wed 11 Apr 2018 16:44:34 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:17019 Wed 11 Apr 2018 15:14:52 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:15131 Wed 11 Apr 2018 12:21:29 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:14316 Wed 11 Apr 2018 09:34:43 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:36671 Tue 23 Jun 2020 12:57:42 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:43108 Tue 13 Sep 2022 13:22:24 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27775 Thu 07 Feb 2019 14:52:24 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:20889 Sat 24 Mar 2018 07:57:55 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:40916 Mon 25 Jul 2022 12:44:17 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:16825 Mon 12 Aug 2024 19:48:50 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:27773 Staphylococcus aureus is an opportunistic pathogen responsible for a high proportion of nosocomial infections. This study was conducted to assess the bacterial responses in the cytoplasmic composition of amino acids and ribosomal proteins under various environmental conditions designed to mimic those on the human skin or within a wound site: pH6-8, temperature 35-37°C, and additional 0-5% NaCl. It was found that each set of environmental conditions elicited substantial adjustments in cytoplasmic levels of glutamic acid, aspartic acid, proline, alanine and glycine (P< 0.05). These alterations generated characteristic amino acid profiles assessed by principle component analysis (PCA). Substantial alterations in cytoplasmic amino acid and protein composition occurred during growth under conditions of higher salinity stress implemented via additional levels of NaCl in the growth medium. The cells responded to additional NaCl at pH 6 by reducing levels of ribosomal proteins, whereas at pH 8 there was an upregulation of ribosomal proteins compared with the reference control. The levels of two ribosomal proteins, L32 and S19, remained constant across all experimental conditions. The data supported the hypothesis that the bacterium was continually responding to the dynamic environment by modifying the proteome and optimising metabolic homeostasis.]]> Fri 05 Aug 2022 15:55:12 AEST ]]>